This volume explores the latest techniques used in the field of mammalian germ cell biology to study key experimental approaches and perspectives for critical analysis of spermatogonial subsets including the rare spermatogonial stem cells (SSCs) pool.  Written in the highly successfulMethods in Molecular Biologyseries format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding common pitfalls.Cutting-edge and comprehensive,Spermatogonial Stem Cells: Methods and Protocolsis a valuable resource for both new and experiences investigators looking to expand their knowledge in this developing field.