This book expands upon the useful first edition by exploring classic Quantitative Polymerase Chain Reaction (qPCR) techniques as well as a number of recently developed applications. With the changes in instrumentation due to technological advances and the development of new reagents to fulfill ethical and legal issues, the qPCR field is now an up-to-date technology that indeed is widely used in research and clinical diagnostics. Written for the highly successfulMethods in Molecular Biologyseries, chapters include introductions to their respective topics, lists of the necessary reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Revised and authoritative,Quantitative Real-Time PCR: Methods and Protocols, Second Editionis an ideal guide to this expanding and vital field of study.