This book explores the integration of nanotechnology, microfabrication, and AI to enhance sensor sensitivity and functionality. It comprehensively covers advancements in lab-on-a-chip, wearable biosensors, and nanozymes, emphasizing their impact on healthcare, food safety, and personalized medicine. The book emphasizes the latest in sensor design, including optical, mechanical, and electrochemical methods and helps in developing learning about nanomaterials, signal amplification strategies, and the role of AI and machine learning in data analysis. The book also addresses the challenges of translating laboratory sensors into market-ready products and outlines essential standards and regulations for biosensor development. It serves as an essential resource for researchers, clinicians, and students, offering insights into the fundamentals and future directions of biosensor technology.