This book constitutes the refereed proceedings of the 6th International Conference on Industrial Applications of Holonic and Multi-Agent Systems, HoloMAS 2013, held in Prague, Czech Republic, in August 2013, in conjunction with DEXA 2013. The 25 revised full papers presented together with two invited talks were carefully reviewed and selected from 37 submissions. The papers are organized in the following topical sections: MAS in automation and manufacturing; design, simulation and validation; MAS in transportation systems; industrial applications; and new trends.