This Special Issue is related to the "2020 International Conference on Biotechnology and Food Science (BFS 2020) " which was held in Guangzhou, China, 18-20 December 2020. BFS 2020 provided an enabling platform for innovative academics, engineers, and industrial experts in the field of biotechnology and food science to exchange new ideas and present research results. Symposium participants, as well as all researchers working in the field, were cordially invited to contribute original research papers or reviews to this Special Issue of the Journal of Fungi .