This book constitutes the refereed proceedings of the 23rd European Conference on Evolutionary Computation in Combinatorial Optimization, EvoCOP 2023, held as part of Evo*2023, in Brno, Czech Republic in April 2023, co-located with the Evo*2023 events: EvoMUSART, EvoApplications, and EuroGP.The 15 revised full papers presented in this book were carefully reviewed and selected from 32 submissions. They present recent theoretical and experimental advances in combinatorial optimization, evolutionary algorithms, and related research fields.