This handbook covers some primary instruments-based techniques used in modern biological science and medical research programs. Key features of the book include introductory notes for each topic, systematic presentation of relevant methods, troubleshooting guides for practical settings. Topics covered in the volume include: Mass spectrometry in proteomics Structural elucidation of biological macromolecules Isothermal titration calorimetry Reverse transcription polymerase chain reaction (RT-PCR) This book is a simple, useful handbook for students and teachers involved in graduate courses in life sciences and medicine. Readers will learn about the basics of featured techniques, the relevant applications and the established protocols.