The two-volume set CCIS 2581 and 2582 constitutes the refereed proceedings of the 26th International Conference on Engineering Applications of Neural Networks , EANN 2025, held in Limassol, Cyprus during June 26&29, 2025. The  41 full  papers included in these proceedings were carefully reviewed and selected from 101 submissions. These papers demonstrate the vitality of Artificial Intelligence algorithms and approaches, as well as AI applications.