The present guide demonstrates the latest achievements in the field of hospital disinfection and sterilization comprising the equipment of a modern central sterile services department, recommendable methods and techniques for reprocessing of medical devices etc. This activity is of exclusive importance for the guarantee of reliable performance of the medical interventions in view of patients' protection against infectious complications, and personnel protection against occupational hazards. The guide is a compilatory work based on novel and approved original works of prominent authors - experts in disinfection and sterilization practices as well as the standpoints and recommendations of competent authorities as professional associations and agencies responsible for quality assessment.