This volume provides a comprehensive study of this key method of gel-based proteomics. Chapters inDifference Gel Electrophoresis: Methods and Protocolsdetail methods on principles of differential protein labeling, two-dimensional gel electrophoresis, techniques on optimized proteomic workflows, advanced mass spectrometry for protein identification, application of basic biological research, and applied biomarker discovery. Written in the highly successfulMethods in Molecular Biologyseries format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Difference Gel Electrophoresis: Methods and Protocolsaims to ensure successful results in the further study of this vital field. | Difference Gel Electrophoresis by Kay Ohlendieck, Hardcover | Indigo Chapters