This book constitutes the proceedings of the 16th International Conference on Remote Engineering and Virtual Instrumentation (REV), held at the BMS College of Engineering, Bangalore, India on 3-6 February 2019. Today, online technologies are at the core of most fields of engineering, as well as of society as a whole, and are inseparably connected with Internet of Things, cyber-physical systems, collaborative networks and grids, cyber cloud technologies, service architectures, to name but a few. Since it was first held in, 2004, the REV conference has focused on the increasing use of the Internet for engineering tasks and the problems surrounding it. The 2019 conference demonstrated and discussed the fundamentals, applications and experiences in the field of online engineering and virtual instrumentation. It also presented guidelines for university-level courses on these topics, in view of the increasing globalization of education and the demand for teleworking, remote services and collaborative working environments.