This edited book is focusing on the novel and innovative procedures in tissue culture for large scale production of plantation and horticulture crops. It is bringing out a comprehensive collection of information on commercial scale tissue culture with the objective of producing high quality, disease-free and uniform planting material. Developing low cost commercial tissue culture can be one of the best possible way to attain the goal of sustainable agriculture. Tissue culture provides a means for rapid clonal propagation of desired cultivars, and a mechanism for somatic hybridization and in vitro selection of novel genotypes. Application of plant tissue culture technology in horticulture and plantation crops provides an efficient method to improve the quality and nutrition of the crops. This book includes a description of highly efficient, low cost in vitro regeneration protocols of important plantation and horticulture crops with a detailed guideline to establish a commercialplant tissue culture facility including certification, packaging and transportation of plantlets. The book discusses somatic embryogenesis, virus elimination, genetic transformation, protoplast fusion, haploid production, coculture of endophytic fungi, effects of light and ionizing radiation as well as the application of bioreactors. This book is useful for a wide range of readers such as, academicians, students, research scientists, horticulturists, agriculturists, industrial entrepreneurs, and agro-industry employees.