This book collects multidisciplinary research articles focused on Clostridioides difficile infection. The contributions collected here shed some light on grey areas of our knowledge of Clostridioides difficile , including regional Clostridioides difficile phenotypic and genotypic patterns, the Clostridioides difficile infection clinical course and the mortality rate in different settings and patient case-mix, the levels of Clostridioides difficile toxins in patients' sera, and novel, promising therapeutic approaches. This collection serves as a valuable reservoir of knowledge for scientists and researchers in the field of infectious diseases.