This volume covers the latest advancements in staining techniques, and protocols and process data using various bioinformatic approaches. The chapters in this book are divided into broad themes of traditional immunohistochemistry, advanced spatial proteomics, and spatial genomics. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, Cellular Spatial Characterization and Analysis is a valuable resource for any researchers looking to learn more about this developing field.