This detailed volume presents hands-on technological protocols used to target an array of cell-secreted extracellular vesicles (EVs) in a variety of biological systems. Beginning with methods for EV purification and analysis, the book continues with sections on the study of EV functions as well as specific systems and models allowing for the study of EVs of different origin. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.  Authoritative and practical, Cell-Secreted Vesicles: Methods and Protocols serves as an ideal guide to conducting systematic assays in an effort to further our understanding of the mode of assembly, secretion, and targeting of EVs which will serve eventually as new therapeutic openings.