This second edition provides updated and new chapters to build on and extend the strengths of the first edition. Chapters guide readers through basic biology of basophils, obtaining the cells by purification, culture of stem cells progenitors, peripheral CD34+stem cell-derived mast cells, basophils from CD34+progenitors, diagnostic applications, gene expression patterns in basophils, roles of basophils in different asthma phenotypes, knockout, and disease models. Written in the highly successfulMethods in Molecular Biologyseries format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Basophils and Mast Cells: Methods and Protocols, Second Editionaims to ensure successful results in the further study of this vital field. | Basophils And Mast Cells by Bernhard F. Gibbs, Hardcover | Indigo Chapters