This detailed book presents state-of-the-art protocols from scientists actively working in autophagy research, a crucial cellular process that regulates numerous cellular functions. The volume explores aspects of autophagy research where a better understanding of its role is vitally important, such as in the development and disease states. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Autophagy in Development and Disease: Methods and Protocols serves as an ideal guide for researchers seeking to expand our knowledge of this key cell biological action.