The adoption of agriculture 4.0 technologies in the agri-food system has been considered a promising alternative to ensure agri-food security and healthy nutrition worldwide. However, it is necessary to clarify which elements help form agriculture 4.0 so that more countries can fully benefit from its potential. "Agriculture 4.0: Towards a New Agri-Food Ecosystem" fills this gap by listing a set of studies on the foundations of agriculture 4.0 in this sector. This book has a didactic and concrete character that can facilitate the understanding of the main elements that are already consolidated in the literature or that are still in the process of being constructed. Although the needs, expectations, and perceptions of agriculture 4.0 may vary between countries and regions, much of the information listed here provides insight into what is fostering a modern agri-food ecosystem anywhere in the world.