The four-volume proceedings LNCS 13090, 13091, 13092, and 13093 constitutes the proceedings of the 27th International Conference on the Theory and Application of Cryptology and Information Security, ASIACRYPT 2021, which was held during December 6-10, 2021. The conference was planned to take place in Singapore, but changed to an online format due to the COVID-19 pandemic. The total of 95 full papers presented in these proceedings was carefully reviewed and selected from 341 submissions. The papers were organized in topical sections as follows: Part I: Best paper awards; public-key cryptanalysis; symmetric key cryptanalysis; quantum security; Part II: physical attacks, leakage and countermeasures; multiparty computation; enhanced public-key encryption and time-lock puzzles; real-world protocols; Part III: NIZK and SNARKs; theory; symmetric-key constructions; homomorphic encryption and encrypted search; Part IV: Lattice cryptanalysis; post-quantum cryptography; advanced encryption and signatures; zero-knowledge proofs, threshold and multi-signatures; authenticated key exchange.