This book consists of the essential contributions presented at the Third International Symposium on Bioinformatics and Biomedicine (BioInfoMed&2024), held in Burgas, Bulgaria, from July 4 to 6, 2024. Bringing together world-renowned scientists and perspective young researchers from 14 countries, the book showcases cutting-edge research at the intersection of bioinformatics, biomedical science, and artificial intelligence. Featuring peer-reviewed contributions on bioinformatics applications, AI in medicine, and technological innovations in healthcare, the book highlights interdisciplinary approaches applied to contemporary challenges in medicine and biology. With insights from prominent leading scientists and a strong focus on emerging technologies, this collection serves as an essential reference for researchers, practitioners, and students in bioinformatics, biomedical engineering, and related fields.