This textbook for undergraduate and postgraduate students discusses advancements in forensic DNA analysis since early texts were published. It presents conventional and latest serological and molecular biological methods for body fluid identification. This book also describes the applications and advantages of next-generation sequencing (NGS) compared to conventional methods in forensic DNA analysis. It also defines the growing importance, techniques, and applications for the analysis of non-human DNA in forensic sciences. Further, the book examines the role of DNA databases in forensic interpretation and criminal investigations. Towards the end, this textbook reviews the application of forensic DNA technology in analyzing real-time casework samples and presents the guidelines, ethical issues, and other challenges of forensic DNA analysis. This textbook is an essential resource for students and practitioners interested in gaining knowledge of up-to-date forensic techniques and their applications in forensic DNA analysis.