This book provides the necessary fundamentals and background for researchers and research professionals working in the field of 3D bioprinting in tissue engineering. In 3D bioprinting, design and development of the biomaterial-inks/bio-inks is a major challenge in providing 3D microenvironments specific to anatomical and architectural demands of native tissues. The focal point of this book is to provide the basic chemistry of biomaterials, updates on current processing, developments, and challenges, and recent advancements in tissue-specific 3D printing/bioprinting. This book is will serve as a go-to reference on bioprinting and is ideal for students, researchers and professionals, working academia, government, the medical industry, and healthcare.